DCTN2 Antibody

• Catalog Number: orb1258223
• Category: Antibodies
• Description: DCTN2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human DCTN2 (NP_006391.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 44kDa
• Target: DCTN2
• UniProt ID: Q13561
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Dynactin subunit 2, 50 kDa dynein-associated polyp
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of MCF-7 cells, using DCTN2 antibody (orb1258223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human kidney using DCTN2 antibody (orb1258223) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using DCTN2 antibody (orb1258223) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using DCTN2 antibody (orb1258223) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using DCTN2 antibody (orb1258223). Blue: DAPI for nuclear staining.