DNA polymerase theta Antibody

SKU: orb20127

Description

Goat polyclonal antibody to DNA Polymerase theta

Research Area

Neuroscience; Molecular Biology

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:1000, WB: 1 μg/ml
• Predicted Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments in Human Brain (Cerebellum lysates) and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

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Key Properties

• Clonality: Polyclonal
• Target: DNA polymerase theta
• Protein Sequence: DTHQQLTCSNDES
• Molecular Weight: 290
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-DKFZp781A0112 antibody, anti-DNA polymerase eta antibody, anti- DNA polymerase theta antibody, anti- DPOLQ_HUMAN antibody, anti- POLH antibody, anti- POLQ antibody, anti- Polymerase (DNA directed) theta antibody, anti- PRO0327 antibody, anti-PolyQ antibody

Quick Database Links

Gene Symbol

DNA polymerase theta

NCBI Gene ID

10721