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Description
Research Area
Cancer, DNA Damage, Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 322-520 of human DNA polymerase eta (NP_006493.1). |
| Molecular Weight | 80kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-DKFZp781A0112 antibody, anti-DNA polymerase eta antibody, anti- DNA polymerase theta antibody, anti- DPOLQ_HUMAN antibody, anti- POLH antibody, anti- POLQ antibody, anti- Polymerase (DNA directed) theta antibody, anti- PRO0327 antibody, anti-PolyQ antibody
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Immunofluorescence analysis of U-2 OS cells using DNA polymerase eta Rabbit pAb (orb48495) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using DNA polymerase eta Rabbit pAb (orb48495) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Pan, Qi et al. Knockdown of POLQ interferes the development and progression of hepatocellular carcinoma through regulating cell proliferation, apoptosis and migration Cancer Cell Int, 21, 482 (2021)
Applications
WB
Reactivity
Human
DNA polymerase eta Rabbit pAb (orb48495)
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