DGAT2 Antibody

• Catalog Number: orb19428
• Category: Antibodies
• Description: Goat polyclonal antibody to DGAT2
• Target: DGAT2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KLEHPTQQDIDLYH
• RRID: AB_10747008
• MW: 43.8; 39.0
• Tested applications: ELISA, FC, IHC
• Dilution range: ELISA: 1:128000, IHC-P: 3 μg/ml, FACS: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3ug/ml.WB: In transfected HEK293 transiently expressing DGAT2 bands of approx. 60kDa and 45kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in Human Liver lysates. The calculated molecular size is 43.8kDa according to NP_115953.2. Recommended concentration, 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DGAT2 antibody, anti diacylglycerol O-acyltra
• Note: For research use only
• Entrez: 84649

References

Mahli, Abdo et al. Bone Morphogenetic Protein-8B Expression is Induced in Steatotic Hepatocytes and Promotes Hepatic Steatosis and Inflammation In Vitro Cells, 8, (2019)

PubMed: 31096638

Immunohistochemical staining of paraffin embedded Human Liver using DGAT2 antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using DGAT2 antibody.