DGAT2 Antibody

SKU: orb19428

Description

Goat polyclonal antibody to DGAT2

Images & Validation

• Tested Applications: ELISA, FC, IHC
• Dilution Range: ELISA: 1:128000, IHC-P: 3 μg/ml, FACS: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3ug/ml.WB: In transfected HEK293 transiently expressing DGAT2 bands of approx. 60kDa and 45kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in Human Liver lysates. The calculated molecular size is 43.8kDa according to NP_115953.2. Recommended concentration, 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DGAT2
• Protein Sequence: KLEHPTQQDIDLYH
• Molecular Weight: 43.8; 39.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DGAT2 antibody, anti diacylglycerol O-acyltransferase homolog 2 (mouse) antibody, anti DKFZp686A15125 antibody, anti HMFN1045 antibody, anti GS1999full antibody, anti diacylglycerol O-acyltransferase homolog 2 antibody, anti diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody

Immunohistochemical staining of paraffin embedded Human Liver using DGAT2 antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using DGAT2 antibody.

Quick Database Links

Gene Symbol

DGAT2

NCBI Gene ID

84649

Mahli, Abdo et al. Bone Morphogenetic Protein-8B Expression is Induced in Steatotic Hepatocytes and Promotes Hepatic Steatosis and Inflammation In Vitro Cells, 8, (2019)

PubMed: 31096638