DGAT2 Antibody

SKU: orb1247609

Description

DGAT2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: EIA, ELISA, FC, IHC
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration: 3ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant DGAT2 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KLEHPTQQDIDLYH
• Target: DGAT2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

DGAT2, diacylglycerol O-acyltransferase homolog 2 (mouse), DKFZp686A15125, HMFN1045 , GS1999full, diacylglycerol O-acyltransferase homolog 2, diacylglycerol O-acyltransferase-like protein 2 isoform 1

orb1247609 (3 ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247609 (0.5 ug/ml) as the reporter antibody.

orb1247609 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

DGAT2

UniProt

Q96PD7

RefSeq (Protein):NP_115953.2, NP_001240820.1