DGAT2 Antibody

• Catalog Number: orb1247609
• Category: Antibodies
• Description: DGAT2 Antibody
• Target: DGAT2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-KLEHPTQQDIDLYH
• UniProt ID: Q96PD7
• Tested applications: EIA, ELISA, FC, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration: 3ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant DGAT2 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DGAT2, diacylglycerol O-acyltransferase homolog 2
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_115953.2, NP_001240820.1

orb1247609 (3 ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247609 (0.5 ug/ml) as the reporter antibody.

orb1247609 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).