DDAH1 Antibody

SKU: orb18846

Description

Goat polyclonal antibody to DDAH1

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:1000, WB: 0.1-0.3 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 37kDa band observed in human kidney, liver and pancreas lysates (calculated MW of 31.1kDa according to NP_036269.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J. 2005 Jul;19(9):1175-7; PMID: 15827267). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DDAH1
• Molecular Weight: 31.1; 20.2; 20.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti DDAH1 antibody, anti dimethylarginine dimethylaminohydrolase 1 antibody, anti DDAH antibody, anti DDAHI antibody, anti NG, NG-dimethylarginine dimethylaminohydrolase antibody, anti RP4-621F18.1 antibody, anti FLJ21264 antibody, anti FLJ25539 antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using DDAH1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using DDAH1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using DDAH1 antibody.

Western blot analysis of Human Cerebellum (A), Kidney (B) and Testes (C) lysate using DDAH1 antibody.

Western blot analysis of HepG2 (A) and LNCaP (B) cell lysate using DDAH1 antibody.

Quick Database Links

Gene Symbol

DDAH1

NCBI Gene ID

23576