DDAH1 Antibody

• Catalog Number: orb1264521
• Category: Antibodies
• Description: DDAH1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This DDAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: DDAH1
• UniProt ID: O94760
• NCBI: O94760
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: N(G),N(G)-dimethylarginine dimethylaminohydrolase
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of DDAH1 Antibody in mouse kidney tissue lysates (35 ug/lane)

Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of DDAH1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).