Goat anti-Cytochrome b reductase 1 Antibody

• Catalog Number: orb18972
• Category: Antibodies
• Description: Goat polyclonal antibody to CYBRD1
• Target: Cytochrome b reductase 1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CRNLALDEAGQRSTM
• RRID: AB_10748131
• MW: 31.6
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.1 μg/ml, IHC-P: 2.5ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 31.6kDa according to NP_079119. The detected bands were all successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CYBRD1 antibody, anti DCYTB antibody, anti FL
• Note: For research use only
• Entrez: 79901

Immunohistochemical staining of Human Testis using CYBRD1 antibody

Immunohistochemical staining of Human Small Intestine using CYBRD1 antibody