NQO1 Rabbit Polyclonal Antibody

• Catalog Number: orb13617
• Category: Antibodies
• Description: NQO1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Equine, Guinea pig, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NQO1 (201-274/274aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: NQO1
• UniProt ID: P15559
• RRID: AB_10749537
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Azoreductase; Cytochrome b 5 reductase; DHQU; DIA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhang, Zhihui et al. Nrf2 antioxidant pathway suppresses Numb-mediated epithelial-mesenchymal transition during pulmonary fibrosis Cell Death Dis, 9, 83 (2018)

PubMed: 29362432

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NQO1) Polyclonal Antibody, Unconjugated (orb13617) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-NQO1 (orb13617) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: MCF-7 (Human) Cell Lysate at 30 ug, Lane 4: Du145 (Human) Cell Lysate at 30 ug, Primary: Anti-NQO1 (orb13617) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Sample: Lane 1: Liver (Rat) Lysate at 30 ug, Lane 2: Brain (Rat) Lysate at 30 ug, Primary: Anti-NQO1 (orb13617) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 31kD, Observed band size: 30kD.

Tissue/Cell: mouse colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NQO1 Polyclonal Antibody, Unconjugated (orb13617) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.