COX4I2 Antibody

• Catalog Number: orb20250
• Category: Antibodies
• Description: Goat polyclonal antibody to COX IV
• Target: COX4I2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RWDYEKKQWKK
• RRID: AB_10746659
• MW: 19.6
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 1-3 μg/ml
• Application notes: This antibody is expected to recognize COX4I1 (GeneID:1327; MW: 19.6kDa) & COX4I2 (GeneID:84701, MW:19.6kDa).ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 17kDa band observed in Human Skeletal Muscle lysates (calculated MW of 19.6kDa according to NP_115998.2). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-AL024441 antibody, anti-COX 4 antibody, anti-
• Note: For research use only
• Entrez: 84701

orb20250 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of staining of Human Skeletal Muscle lysate using COX4I2/COX4I1 antibody