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| Catalog Number | orb770652 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to COX IV |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human COX42. AA range:31-80 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 20 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-COX4I2 antibody, anti-COX4L2 antibody, anti-C Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of K562 insulin 0.01U/ml 15' cells using COX4I2 Polyclonal Antibody.
Immunofluorescence analysis of COS7 cells, using COX42 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from K562 cells, treated with insulin 0.01U/ml 15', using COX42 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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