Clusterin/APOJ Antibody

SKU: orb19080

Description

Goat polyclonal antibody to CLU

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35-37kDa band observed in human colon, kidney and prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 9713325 and PMID: 11560857). Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Clusterin / APOJ
• Protein Sequence: EKALQEYRKKHREE
• Molecular Weight: 57.8; 52.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Clusterin antibody, anti CLU antibody, anti APOJ antibody, anti CLI antibody, anti KUB1 antibody, anti Apolipoprotein J antibody, anti Apo-J antibody

Western blot analysis of human colon lysate using CLU antibody

Immunohistochemical staining of Human Testis using CLU antibody

Immunofluorescence analysis of HeLa cells using CLU antibody

Quick Database Links

Gene Symbol

Clusterin / APOJ

NCBI Gene ID

1191