Goat anti-Clusterin / ApoJ (mouse) Antibody

SKU: orb20050

Description

Goat polyclonal antibody to CLU

Research Area

Apoptotic, Cancer, Epigenetics, Immunology, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 37kDa band observed in Mouse Brain and Testis lysates and in Rat Brain and Ovary lysates (calculated MW of 51.6kDa according to Mouse NP_038520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104(12):1407-12.; PMID: 11560857). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Clusterin / ApoJ (mouse)
• Protein Sequence: EKALQEYRRKSRAE
• Molecular Weight: 51.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Clusterin antibody, anti CLU antibody, anti APOJ antibody, anti CLI antibody, anti KUB1 antibody, anti Apolipoprotein J antibody, anti Apo-J antibody

Western blot analysis of Rat Brain lysate using CLU antibody

Western blot analysis of Rat Brain lysate using CLU antibody

Western blot analysis of Human Testes lysate using CLU antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using CLU antibody

Quick Database Links

Gene Symbol

Clusterin / ApoJ (mouse)