Goat anti-CDYL Antibody

• Catalog Number: orb18440
• Category: Antibodies
• Description: Goat polyclonal antibody to CDYL
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 60.6; 45.1
• Target: CDYL
• Entrez: 9425
• Protein Sequence: SMLKYLQRKIDEF
• RRID: AB_10750567
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CDYL antibody, anti chromodomain protein, Y-l
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 75kDa band observed in Human Testes lysates (calculated MW of 66.5kDa according to NP_004815.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8707-12; PMID: 12072557). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb18440 staining (1ug/ml) of Human Testes lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.