CCKBR Antibody

• Catalog Number: orb19186
• Category: Antibodies
• Description: Goat polyclonal antibody to Gastrin Receptor
• Target: CCKBR
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: FDGDSDSDSQSRVRNQ
• RRID: AB_10748646
• MW: 48.4
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows strong staining in islets of Langerhans and weak staining in acinar cells. Recommended concentration, 3ug/ml.WB: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J, Int J Endocrinol. 2009;2009:875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-G14 antibody, anti-G17 antibody, anti-G34 ant
• Research Area: Cancer, Epigenetics, GPCR, Neuroscience, Signaling
• Note: For research use only
• Entrez: 887

Immunohistochemical staining of Human Placenta using CCKBR antibody

Western blot analysis Mouse Pancreas lysate using CCKBR antibody

Immunohistochemical staining of Human Pancreas using CCKBR antibody

Immunohistochemical staining of Human Uterus using CCKBR antibody

Western blot analysis of Mouse Pancreas(lane 1) and Rat Stomach(lane 2) lysate using CCKBR antibody

Immunohistochemical staining of Human Pancreas using CCKBR antibody

Immunohistochemical staining of Pancreas using CCKBR antibody