CCKBR Antibody

SKU: orb1247691

Description

CCKBR Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 80kDa band observed in Mouse Pancreas and Rat Stomach lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1 and 49.0kDa according to Rat NP_037297.1). The observed molecular weight also corresponds to earlier findings in literature with different antibodies. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml. In paraffin embedded Human Pancreas expression of CCKBR is detected in islet cells, and not in surrounding acinar cells. Recommended concentration: 6ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-FDGDSDSDSQSRVRNQ
• Target: CCKBR
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

CCKBR, cholecystokinin B receptor , HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrin\cholecystokinin brain receptor, CCK2R

orb1247691 (1 ug/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247691 (5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247691 (6 ug/ml) staining of paraffin embedded Human Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247691 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.

Quick Database Links

Gene Symbol

CCKBR

UniProt

P32239

RefSeq (Protein):NP_795344.1