Caspase 3 Antibody

SKU: orb125009

Description

Goat polyclonal antibody to Caspase 3

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 0.5-1 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  WB: Approx 32kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6kDa according to Human NP_004337.2 and 31.4kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65kDa was also consistently ob. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.

Key Properties

• Clonality: Polyclonal
• Target: Caspase 3
• Protein Sequence: RDVSKEDHSKRS
• Molecular Weight: 31.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Apopain antibody, anti CASP 3 antibody, anti CASP-3 antibody, anti CASP3 antibody, anti CC3 antibody, anti CPP 32 antibody, anti CPP-32 antibody, anti CPP32 antibody, anti CPP32B antibody, anti ICE3 antibody, anti LICE antibody, anti PARP cleavage protease antibody, anti Procaspase3 antibody, anti Protein Yama antibody, anti Yama antibody, anti Yama protein antibody, anti Caspase-3 antibody, anti Caspase3 antibody, anti caspase3 antibody, anti caspase 3 antibody

Western blot analysis of MOLT4 lysate using Caspase 3 antibody

Western blot analysis of Mouse Liver lysate using Caspase 3 antibody

Quick Database Links

Gene Symbol

Caspase 3

NCBI Gene ID

836