BNIP1 Antibody

SKU: orb19606

Description

Goat polyclonal antibody to BNIP1

Research Area

Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 0.5-1.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 28kDa band observed in Human Kidney lysates (calculated MW of 26.1kDa according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BNIP1
• Protein Sequence: DLLRQRKTTKESLAQ
• Molecular Weight: 26.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BNIP1 antibody, anti adenovirus E1B 19kDa interacting protein 1 antibody, anti NIP1 antibody, anti SEC20 antibody, anti TRG-8 antibody, anti adenovirus E1B 19kD interacting protein 1 antibody, anti adenovirus E1B 19kD-interacting protein 1 antibody

orb19606 (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

BNIP1

NCBI Gene ID

662