BNIP1 Antibody

• Catalog Number: orb19606
• Category: Antibodies
• Description: Goat polyclonal antibody to BNIP1
• Target: BNIP1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DLLRQRKTTKESLAQ
• RRID: AB_10749635
• MW: 26.1
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.5-1.5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 28kDa band observed in Human Kidney lysates (calculated MW of 26.1kDa according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BNIP1 antibody, anti adenovirus E1B 19kDa int
• Research Area: Apoptotic, Cancer, Epigenetics, Infectious Disease
• Note: For research use only
• Entrez: 662

orb19606 (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.