NIP1 Antibody (BH3 Domain Specific)

• Catalog Number: orb1936887
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB1274
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 26132 Da
• Target: This NIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-127 amino acids from human NIP1.
• UniProt ID: Q12981
• NCBI: NP_001196.2, NP_053581.2, NP_053582.2, NP_053583.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Vesicle transport protein SEC20, BCL2/adenovirus E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-NIP1 BH3 Domain Pab in HL60 cell lysates (35 ug/lane). NIP1 BH3 Domain (arrow) was detected using the purified Pab.