Biglycan Preproprotein Antibody

SKU: orb19394

Description

Goat polyclonal antibody to BGN

Images & Validation

• Tested Applications: ELISA, IF
• Dilution range: ELISA: 1:2000, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 38-40kDa band observed in Rat Skin lysates (calculated MW of 41.7kDa according to rat NP_058783.1 and human NP_001702.1 ). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Biglycan Preproprotein
• Molecular Weight: 41.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration:

  0.1-0.3 μg/ml

• Disclaimer: For research use only

Alternative Names

anti Biglycan Preproprotein antibody, anti biglycan antibody, anti BGN antibody, anti DSPG1 antibody, anti PG-S1 antibody, anti PGI antibody, anti SLRR1A antibody, anti bone/cartilage proteoglycan-I antibody, anti dermatan sulphate proteoglycan I antibody, anti small leucine-rich protein 1A antibody

Immunofluorescence analysis of NIH3T3 cells using BGN antibody.

Immunofluorescence analysis of HepG2 cells using BGN antibody.

Quick Database Links

Gene Symbol

Biglycan Preproprotein

NCBI Gene ID

633

References

Burssens, A. et al. Arguments for an increasing differentiation towards fibrocartilaginous components in midportion Achilles tendinopathy Knee Surg Sports Traumatol Arthrosc, 21, 1459-1467 (2013)

PubMed: 23001016

Applications

IHC

Reactivity

Human