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| Tested Applications | ELISA, IF |
|---|---|
| Dilution Range | ELISA: 1:2000, IHC-P: 10 μg/ml |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Canine, Rat |
| Application Notes |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | Biglycan Preproprotein |
| Protein Sequence | DRLAIQFGNYKK |
| Molecular Weight | 41.7 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Concentration | 0.1-0.3 μg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti Biglycan Preproprotein antibody, anti biglycan antibody, anti BGN antibody, anti DSPG1 antibody, anti PG-S1 antibody, anti PGI antibody, anti SLRR1A antibody, anti bone/cartilage proteoglycan-I antibody, anti dermatan sulphate proteoglycan I antibody, anti small leucine-rich protein 1A antibody
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Protocol Information
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Burssens, A. et al. Arguments for an increasing differentiation towards fibrocartilaginous components in midportion Achilles tendinopathy Knee Surg Sports Traumatol Arthrosc, 21, 1459-1467 (2013)
Applications
IHC
Reactivity
Human


