BGN Antibody

SKU: orb1247732

Description

BGN Antibody

Images & Validation

• Tested Applications: ELISA, IF
• Reactivity: Mouse
• Predicted Reactivity: Canine, Human, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:2000.Immunofluorescence: Expression of the protein seen in the Golgi of NIH3T3 and HepG2 cells. Recommended concentration: 10µg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DRLAIQFGNYKK
• Target: BGN
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

Biglycan Preproprotein, biglycan, BGN, DSPG1, PG-S1, PGI, SLRR1A , bone/cartilage proteoglycan-I, dermatan sulphate proteoglycan I, small leucine-rich protein 1A

orb1247732 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

BGN

UniProt

P21810

RefSeq (Protein):NP_001702.1