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Goat anti-BHMT Antibody

Catalog Number: orb19599

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19599
CategoryAntibodies
DescriptionGoat polyclonal to BHMT
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityCanine, Human, Mouse, Rat
Dilution rangeELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
ConjugationUnconjugated
MW44.9
TargetBHMT
Entrez635
Protein SequenceEQQLKELFEKQK
RRIDAB_10748411
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti BHMT antibody, anti betaine-homocysteine meth
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4ug/ml.WB: Approx 45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.
Expiration Date12 months from date of receipt.
Goat anti-BHMT Antibody

orb19599 (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat anti-BHMT Antibody

HEK293 overexpressing BHMT RC203148) and probed with orb19599 (mock transfection in first lane), tested by Origene.

Goat anti-BHMT Antibody

Immunohistochemical staining of Human Liver using BHMT antibody

Goat anti-BHMT Antibody

Immunohistochemical staining of Human Kidney using BHMT antibody