BHMT Antibody

SKU: orb19599

Description

Goat polyclonal to BHMT

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4ug/ml.WB: Approx 45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BHMT
• Molecular Weight: 44.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti BHMT antibody, anti betaine-homocysteine methyltransferase antibody

orb19599 (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing BHMT RC203148) and probed with orb19599 (mock transfection in first lane), tested by Origene.

Immunohistochemical staining of Human Liver using BHMT antibody

Immunohistochemical staining of Human Kidney using BHMT antibody

Quick Database Links

Gene Symbol

BHMT

NCBI Gene ID

635