BAALC Antibody

• Catalog Number: orb19109
• Category: Antibodies
• Description: Goat polyclonal antibody to BAALC
• Target: BAALC
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Human, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KQNGLQTTEAKRDAK
• RRID: AB_10767265
• MW: 15.6
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 18kDa band observed in mouse brain lysates (calculated MW of 15.6kDa according to human NP_079088.1 and of 15.5kDa according to mouse NP_542371.1. Recommended concentration: 0.03-0.1 μg/ml. An approx, 48kDa band was consistently observed in both Human and Mouse Brain lysates. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BAALC antibody, anti brain and acute leukemia
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 79870

orb19109 (0.03ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing BAALC (RC203701) and probed with orb19109 (mock transfection in first lane), tested by Origene.