BAALC Antibody

• Catalog Number: orb1247746
• Category: Antibodies
• Description: BAALC Antibody
• Target: BAALC
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: The immunogen for this antibody is: C-KQNGLQTTEAKRDAK
• UniProt ID: Q8WXS3
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 18kDa band observed in mouse brain lysates (calculated MW of 15.6kDa according to human NP_079088.1 and of 15.5kDa according to mouse NP_542371.1. Recommended concentration: 0.03-0.1ug/ml. An approx, 48kDa band was consistently observed in both Human and Mouse Brain lysates. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BAALC, brain and acute leukemia, cytoplasmic , HGN
• Note: For research use only
• NCBI: NP_079088.1

orb1247746 (0.03 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing BAALC and probed with orb1247746 (mock transfection in first lane).