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Item 1 of 2
| Catalog Number | orb178380 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to ATP5A1 |
| Target | ATP5A1 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Protein Sequence | RVHGLRNVQAEE |
| MW | 59.8; 57.5; 54.5 |
| Tested applications | ELISA, WB |
| Dilution range | ELISA: 1:128000, WB: 0.01-0.03 μg/ml |
| Application notes | WB: Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti ATP synthase, H+ transporting, mitochondrial Read more... |
| Note | For research use only |
| Entrez | 498 |
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Sikorski, Krzysztof et al. A high-throughput pipeline for validation of antibodies Nat Methods, 15, 909-912 (2018)
Applications
WB
Western blot analysis of taining of staining of Pig Heart lysates using ATP5A1 antibody.
Western blot analysis of staining of staining of Human (A), fetal Mouse (B), adult Mouse (C) and adult Rat (D) Heart lysates using ATP5A1 antibody.
