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ATP5A1 Antibody

SKU: orb178380
FeaturedFeatured Product

Description

Goat polyclonal antibody to ATP5A1

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeELISA: 1:128000, WB: 0.01-0.03 μg/ml
ReactivityHuman, Mouse, Porcine, Rat
Predicted ReactivityBovine, Canine
Application Notes
WB: Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

HostGoat
ClonalityPolyclonal
TargetATP5A1
Molecular Weight59.8; 57.5; 54.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody, anti ATP5A antibody, anti ATP5AL2 antibody, anti ATPM antibody, anti MOM2 antibody, anti hATP1 antibody, anti ATP synthase alpha chain, mitochondrial antibody, anti ATP synthase subunit alpha, mitochondrial antibody, anti ATP synthase, H+ transportin antibody

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ATP5A1 Antibody

Western blot analysis of taining of staining of Pig Heart lysates using ATP5A1 antibody.

ATP5A1 Antibody

Western blot analysis of staining of staining of Human (A), fetal Mouse (B), adult Mouse (C) and adult Rat (D) Heart lysates using ATP5A1 antibody.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Filter by Applications

Sikorski, Krzysztof et al. A high-throughput pipeline for validation of antibodies Nat Methods, 15, 909-912 (2018)

Applications

WB

ATP5A1 Antibody (orb178380)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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