ATP5A1 Antibody

SKU: orb1257272

Description

ATP5A1 Antibody

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).
• Target: ATP5A1
• Molecular Weight: Observed: 53kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

ATP5A1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1, ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transportin

Western blot analysis of extracts of various cell lines, using ATP5A1 Antibody (orb1257272) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat ovary using ATP5A1 antibody (orb1257272) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using ATP5A1 antibody (orb1257272) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ATP5A1 antibody (orb1257272) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using ATP5A1 antibody (orb1257272) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ATP5A1 antibody (orb1257272) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using ATP5A1 antibody (orb1257272) at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using ATP5A1 antibody (orb1257272) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells using 1 ug ATP5A1 antibody (orb1257272). Western blot was performed from the immunoprecipitate using ATP5A1 antibody (orb1257272) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

ATP5A1

UniProt

P25705