ATP5A1 Antibody

• Catalog Number: orb1257272
• Category: Antibodies
• Description: ATP5A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 53kDa
• Target: ATP5A1
• UniProt ID: P25705
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ATP5A1, ATP synthase, H+ transporting, mitochondri
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ATP5A1 Antibody (orb1257272) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat ovary using ATP5A1 antibody (orb1257272) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using ATP5A1 antibody (orb1257272) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ATP5A1 antibody (orb1257272) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using ATP5A1 antibody (orb1257272) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ATP5A1 antibody (orb1257272) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using ATP5A1 antibody (orb1257272) at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using ATP5A1 antibody (orb1257272) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells using 1 ug ATP5A1 antibody (orb1257272). Western blot was performed from the immunoprecipitate using ATP5A1 antibody (orb1257272) at a dilution of 1:1000.