Goat anti-ATG16L1 Antibody

• Catalog Number: orb19825
• Category: Antibodies
• Description: Goat polyclonal antibody to ATG16L1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.01-0.1 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 68.3, 66.3
• Target: ATG16L1
• Entrez: 55054
• Protein Sequence: KVEKVLSKQHSSSIN
• RRID: AB_10754198
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ATG16 antibody, anti autophagy related 16-lik
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 48kDa band in Human Kidney, Liver and Spleen lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.3kDa according to NP_110430.5 and of 66.3kDa according to NP_060444.3. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse Brain lysate using ATG16L1 antibody

Immunohistochemical staining of Human Skin using ATG16L1 antibody