Goat anti-Arginase I Antibody

• Catalog Number: orb20124
• Category: Antibodies
• Description: Goat polyclonal antibody to ARG1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:64000, WB: 0.01-0.03 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 34.7
• Target: Arginase I
• Entrez: 383
• Protein Sequence: CFGLAREGNHKPID
• RRID: AB_10768330
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ARG1 antibody, anti arginase, liver antibody,
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney shows preferential staining of PCT. Recommended concentration, 2-4µg/ml.WB: Approx. 37kDa band observed in Human Liver lysates (calculated MW of 34.7kDa according to NP_000036.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

0.01 μg/ml staining of Human (A) and (0.03 μg/ml) Pig (B) Liver lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

3.75 μg/ml staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

0.3 μg/ml staining of HepG2 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.