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Arginase 1 Rabbit Polyclonal Antibody

Catalog Number: orb1265

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1265
CategoryAntibodies
DescriptionArginase 1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Canine, Mouse, Rabbit, Sheep
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Arginase 1 (151-220/322aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
Form/AppearanceLiquid
ConjugationUnconjugated
MW35 kDa
TargetARG1
UniProt IDP05089
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesliver Arginase; ARG 1; ARG1; ARGI1_HUMAN; Arginase
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NoteFor research use only
Expiration Date12 months from date of receipt.
Arginase 1 Rabbit Polyclonal Antibody

Blank control (blue): Hep G2 cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-CD59 antibody (orb1265), Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Arginase 1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Arginase 1) Polyclonal Antibody, Unconjugated (orb1265) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Arginase 1 Rabbit Polyclonal Antibody

Tissue/Cell: human liver carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Arginase 1 Polyclonal Antibody, Unconjugated (orb1265) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • Arginase 1 antibody [orb556594]

    ELISA,  FACS,  ICC,  IHC-Fr,  IHC-P,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Arginase 1 Rabbit Polyclonal Antibody [orb499681]

    IF,  IHC-Fr,  IHC-P,  WB

    Canine, Mouse, Porcine, Rabbit

    Human, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • Arginase I Polyclonal Antibody [orb1414626]

    IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Arginase I rabbit pAb [orb768903]

    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • ARG1 Antibody [orb628462]

    ELISA,  FC,  IF,  IHC,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg