AKAP10 Antibody

• Catalog Number: orb19929
• Category: Antibodies
• Description: Goat polyclonal antibody to AKAP10
• Target: AKAP10
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RKVKGKEQEKTSD
• RRID: AB_10922719
• MW: 73.8
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 74kDa band observed in Rat Brain lysates (calculated MW of 73.8kDa according to Human NP_009133.2 and according to Rat NP_001108078.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti AKAP10 antibody, anti A kinase (PRKA) anchor
• Note: For research use only
• Entrez: 11216

orb19929 (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.