AES Antibody

• Catalog Number: orb22478
• Category: Antibodies
• Description: Goat Polyclonal to AES
• Target: AES
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SHLPQQLKFTTSD
• RRID: AB_10919017
• MW: 29.1; 22.0; 21.8
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: WB: Approx 30kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 29.1kDa according to NP_945320.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti -amino enhancer of split antibody, anti-TLE5
• Research Area: Epigenetics, Neuroscience, Signaling Pathways, Wnt
• Note: For research use only
• Entrez: 166
• Expiration Date: 12 months from date of receipt.

orb22478 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.