AES/Groucho Antibody

• Catalog Number: orb1538323
• Category: Antibodies
• Description: AES/Groucho Antibody
• Target: AES / Groucho
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 16 AA synthetic peptide near the C-Terminus of human AES. The immunogen is located within the last 50 AA of AES.
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (2.5 µg/ml), WB (1 - 2 µg/ml)
• Application notes: Further information: AES antibody can be used for the detection of AES by Western blot at 1 - 4 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AES, AES-1, AES-2, Amino enhancer of split, ESP1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.

Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.

Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.