GNL3 Antibody (N-term)

• Catalog Number: orb1926563
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-124 amino acids from the N-terminal region of human GNL3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9BVP2
• MW: 61993 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:25, WB: 1:2000
• Clone Number: RB51601
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Guanine nucleotide-binding protein-like 3, E2-indu
• Research Area: Stem Cell & Developmental Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-GNL3 Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: NCCIT whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 62 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling GNL3 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing both nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin at 1/100 dilution (red).