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Catalog Number | orb1788084 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 38-72 amino acids from the N-terminal region of human GNL3. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Predicted Reactivity | Rat |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Immunogen | 38-72 aa |
UniProt ID | Q9BVP2 |
MW | 61993 Da |
Tested applications | IF, WB |
Dilution range | IF: 1:25, WB: 1:1000 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Guanine nucleotide-binding protein-like 3, E2-indu Read more... |
Research Area | Stem Cell & Developmental Biology |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Fluorescent image of Hela cells stained with GNL3 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). DAPI was used to stain the cell nuclear (blue).
All lanes: Anti-GNL3 Antibody (N-term) at 1:1000 dilution. Lane 1: F9 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: K562 whole cell lysates. Lane 4: Raji whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 62 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.