GNAI3 Antibody

SKU: orb1274512

Description

GNAI3 Antibody

Research Area

Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IP, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).
• Target: GNAI3
• Molecular Weight: Observed: 45kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Gai3

Western blot analysis of extracts of various cell lines, using GNAI3 antibody (orb1274512) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts from normal (control) and GNAI3 knockout (KO) HeLa cells, using GNAI3 antibody (orb1274512) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug GNAI3 antibody (orb1274512). Western blot was performed from the immunoprecipitate using GNAI3 antibody (orb1274512) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

GNAI3

UniProt

P08754