Csf2 Antibody (Biotin)

SKU: orb1671456

Description

Csf2 Antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse
• Application Notes:
  Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Murine GM-CSF as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mGM-CSF. Western Blot To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Murine GM-CSF. Anti-Murine GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated.
• Target: Csf2
• Purification: GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-alpha

To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine GM-CSF as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mGM-CSF.

To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

Csf2

UniProt

P01587

RefSeq:P01587