CSF2 Antibody (Biotin)

SKU: orb1272524

Description

CSF2 Antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Sandwich:To detect hGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human GM-CSF (XP-5144) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF. Western Blot:To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF (human Granulocyte Macrophage Colony Stimulating Factor).
• Target: CSF2
• Purification: Anti-hGM-CSF specific antibody was purified by affinity chromatography and then biotinylated.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

GMCSF, GMCSF, Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, GM-CSF

To detect hGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human GM-CSF (orb1272525) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGM-CSF.

Quick Database Links

Gene Symbol

CSF2

UniProt

P04141

RefSeq:P04141