Glutamine Synthetase Antibody / GLUL

• Catalog Number: orb1824336
• Category: Antibodies
• Description: Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Canine, Human
• Immunogen: A recombinant partial protein sequence (within amino acids 50-250) from the human protein was used as the immunogen for the Glutamine Synthetase antibody.
• UniProt ID: P15104
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: GLUL/6601
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Glutamine Synthetase antibody is available for research use only.
• Note: For research use only

Western blot testing of human liver tissue lysate with Glutamine Synthetase antibody (clone GLUL/6601). Predicted molecular weight ~42 kDa.

Western blot testing of human Y79 cell lysate with Glutamine Synthetase antibody (clone GLUL/6601). Predicted molecular weight ~42 kDa.

IHC staining of FFPE human stomach tissue with Glutamine Synthetase antibody (clone GLUL/6601) at 2 ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE dog liver with Glutamine Synthetase antibody (clone GLUL/6601). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.