GluN1 Antibody

SKU: orb67498

Description

Mouse Anti-Rat GluN1 Monoclonal IgG1

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-410 was sufficient for detection of NR1 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N308/48
• Immunogen: Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1
• Target: GluN1
• Molecular Weight: 105kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GRIN1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, Glutamate receptor ionotropic N methyl D aspartate 1, MRD8, Methyl-D-aspartate receptor subunit zeta-1, N methyl D aspartate receptor, N methyl D aspartate receptor channel subunit zeta 1, N methyl D aspartate receptor subunit NR1, NMD-R1, NMDA 1, NMDA R1, NMDA Receptor 1, NMDA1, NMDAR, NMDAR1, NMDZ1_HUMAN, NR1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN1/NR1 Monoclonal Antibody, Clone N308/48. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN1/NR1 Antibody (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone N308/48. Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

GluN1

UniProt

P35439

NCBI Gene ID

24408

RefSeq (Protein):NP_058706.1