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NMDAR1 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb704351

DispatchUsually dispatched within 5-10 working days
$ 200.00
Catalog Numberorb704351
CategoryAntibodies
DescriptionNMDAR1 Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone Number13C4
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityRat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant human NMDAR1
Antibody TypeRecombinant Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW103 kDa
TargetGRIN1
UniProt IDQ05586
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesNMDA-NR1; N-Methyl-d-Asprtate receptor 1; GRIN1; N
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NoteFor research use only
Expiration Date12 months from date of receipt.
NMDAR1 Recombinant Rabbit Monoclonal Antibody

N2A cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (NMDAR1) monoclonal Antibody, Unconjugated (orb704351) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

NMDAR1 Recombinant Rabbit Monoclonal Antibody

Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-NMDAR1 antibody (orb704351), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

NMDAR1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cerebellum), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NMDAR1) Monoclonal Antibody, Unconjugated (orb704351) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NMDAR1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NMDAR1) Monoclonal Antibody, Unconjugated (orb704351) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NMDAR1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NMDAR1) Monoclonal Antibody, Unconjugated (orb704351) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NMDAR1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (NMDAR1) Monoclonal Antibody, Unconjugated (orb704351) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NMDAR1 Recombinant Rabbit Monoclonal Antibody

SHG-44 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (NMDAR1) monoclonal Antibody, Unconjugated (orb704351) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

NMDAR1 Recombinant Rabbit Monoclonal Antibody

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (NMDAR1) monoclonal Antibody, Unconjugated (orb704351) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.