GLUL Antibody (N-term)

SKU: orb1788054

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:25
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This GLUL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-96 amino acids from the N-terminal region of human GLUL. Antigen Region: 70-96 aa.
• Target: GLUL {ECO:0000303|PubMed:30158707, ECO:0000312|HGNC:HGNC:4341}
• Molecular Weight: 42064 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glutamine synthetase, GS, Glutamate decarboxylase, Glutamate--ammonia ligase, GLUL, GLNS

Immunohistochemical analysis of paraffin-embedded H. liver section using GLUL Antibody (N-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-GLUL Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: HT-29 whole cell lysates. Lane 3: Jurkat whole cell lysates. Lane 4: MOLT-4 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GLUL Antibody (N-term) at 1:1000 dilution. Lane 1: mouse brain lysate. Lane 2: mouse cerebellum lysate. Lane 3: rat cerebellum lysate. Lane 4: human brain lysate. Lane 5: human cerebellum lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GLUL Antibody (N-term) at 1:1000 dilution. Lane 1: mouse brain lysates. Lane 2: mouse cerebellum lysates. Lane 3: rat cerebellum lysates. Lane 4: human brain lysates. Lane 5: human cerebellum lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

GLUL {ECO:0000303|PubMed:30158707, ECO:0000312|HGNC:HGNC:4341}

UniProt

P15104

RefSeq (Protein):NP_002056.2, NP_001028228.1, NP_001028216.1