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Item 1 of 3
GLUD1 / GLUD2 Antibody
Catalog Number: orb1249164
| Catalog Number | orb1249164 |
|---|---|
| Category | Antibodies |
| Description | GLUD1 / GLUD2 Antibody |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC, WB |
| Predicted Reactivity | Bovine, Canine |
| Reactivity | Human, Mouse, Rat |
| Immunogen | The immunogen for this antibody is: C-ESEEQKRNRVRGILR |
| Antibody Type | Primary Antibody |
| Concentration | 500 ug/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| Target | GLUD1 / GLUD2 |
| UniProt ID | P00367 |
| NCBI | NP_036216.2, NP_005262.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | GLUD1, glutamate dehydrogenase 1, GDH, GDH1, GLUD, Read more... |
| Note | For research use only |
| Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5ug/ml.Immunofluorescence: Customer found particulate cytoplasm staining in HeLa. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
| Expiration Date | 12 months from date of receipt. |
orb1249164 (0.03 ug/ml) staining of Human (A) and Mouse (B) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1249164 (3.75 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1249164 (0.01 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
