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Glutamate Dehydrogenase Antibody

SKU: orb20463

Description

Goat polyclonal antibody to GLUD1 / GLUD2

Research Area

Neuroscience; Metabolism Research

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3-5 μg/ml
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine
Application Notes
IHC: In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration, 3-5μg/ml.WB: Approx 55kDa band observed in Human Liver and in Mouse Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1 and NP_036216.2 and of 61.3 according to Mouse NP_032159.1. Recommended concentration: 0.3-1 μg/ml.

Key Properties

ClonalityPolyclonal
TargetGlutamate Dehydrogenase
Protein SequenceESEEQKRNRVRGILR
Molecular Weight61.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti GDH antibody, anti GDH1 antibody, anti GLUD antibody, anti glutamate dehydrogenase 1 antibody, anti MGC132003 antibody, anti GLUD1 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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