Glutamate Dehydrogenase Antibody

• Catalog Number: orb20463
• Category: Antibodies
• Description: Goat polyclonal antibody to GLUD1 / GLUD2
• Target: Glutamate Dehydrogenase
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ESEEQKRNRVRGILR
• RRID: AB_10748971
• MW: 61.4
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3-5 μg/ml
• Application notes: IHC: In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration, 3-5μg/ml.WB: Approx 55kDa band observed in Human Liver and in Mouse Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1 and NP_036216.2 and of 61.3 according to Mouse NP_032159.1. Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti GDH antibody, anti GDH1 antibody, anti GLUD a
• Note: For research use only
• Entrez: 2746, 2747
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse liver lysate (35µg protein in RIPA buffer) using Glutamate Dehydrogenase antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human cerebral cortex tissue using Glutamate Dehydrogenase antibody

Western blot analysis of Human (Lane1), Mouse (Lane2) and Rat (Lane3) Liver lysate using GLUD1 / GLUD2 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Cerebral Cortex tissue using GLUD1 / GLUD2 antibody

Western blot analysis of Human (Lane1) and Mouse (Lane2) Brain lysate using GLUD1 / GLUD2 antibody