GLS2 Antibody

• Catalog Number: orb1925617
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This GLS2 antibody is generated from a mouse immunized with a recombinant protein of human GLS2.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: Q9UI32
• MW: 66323 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:25
• Clone Number: 1758CT879.217.60
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Glutaminase liver isoform, mitochondrial, GLS, 3.5
• Note: For research use only

All lanes: Anti-GLS2 Antibody at 1:1000-1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.