GLS2 Antibody

• Catalog Number: orb1239416
• Category: Antibodies
• Description: GLS2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2.The immunogen is located within amino acids 300 - 350 of GLS2.
• Concentration: 1 mg/mL
• Dilution range: GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa
• Target: GLS2
• UniProt ID: Q9UI32
• NCBI: NP_037399
• Storage: GLS2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: GLS2 Antibody: GA, GLS, LGA, hLGA, GA, Glutaminase
• Note: For research use only
• Application notes: GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GLS2 in rat liver tissue lysate with GLS2 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of GLS2 in rat liver tissue with GLS2 antibody at 5 µg/mL.

Immunofluorescence of GLS2 in rat liver cells with GLS2 antibody at 20 5 µg/mL.

Immunohistochemistry of GLS2 in mouse brain tissue with GLS2 Antibody at 5 µg/mL.

Immunofluorescence of GLS2 in mouse brain tissue with GLS2 Antibody at 20 µg/mL. Green: GLS2 antibody (orb1239416) Red: Phylloidin staining Blue: DAPI staining.