GLS1 Rabbit Polyclonal Antibody

SKU: orb157166

Description

GLS1 Rabbit Polyclonal Antibody

Research Area

Amino Acid Metabolism, Glutamate, Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GLS1 (141-240/669aa)
• Target: GLS
• Molecular Weight: 72 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GLSK_HUMAN; Glutaminase kidney isoform, mitochondrial; GLS; EC:3.5.1.2; K-glutaminase; L-glutamine amidohydrolase; GLS1; KIAA0838; GAC; GAM; KGA; AAD20; DEE71; GDPAG; CASGID; EIEE71;

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLS1) Polyclonal Antibody, Unconjugated (orb157166) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLS1) Polyclonal Antibody, Unconjugated (orb157166) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human U251 cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-GLS1 (orb157166) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72 kDa, Observed band size: 75 kDa.

Quick Database Links

Gene Symbol

GLS

UniProt

O94925