GITRL Antibody

SKU: orb1239752

Description

GITRL Antibody

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Reactivity: Human, Mouse
• Application Notes:
  GITRL antibody can be used for the detection of GITRL by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. Antibody validated: Immunocytochemistry in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: GITRL antibody was raised against purified recombinant human GITR ligand.
• Target: TNFSF18
• Molecular Weight: Predicted: 23 kD Observed: 27 kD
• Purification: GITRL Antibody is Protein A purified.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: GITRL Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

GITRL Antibody: TL6, AITRL, GITRL, hGITRL, TL6, UNQ149/PRO175, Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand

WB Validation in Human Pancreas. Loading: 10 µg of lysate Antibodies: GITRL, orb1239752, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Spleen. Loading: 10 µg of lysate Antibodies: GITRL, orb1239752, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation with Recombinant Protein. Loading: GITRL recombinant protein lysate Antibodies: GITRL, orb1239752, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Lane A: 5 ng GITRL Protein Lane B: 25 ng GITRL Protein Lane C: 50 ng GITRL Protein.

Quick Database Links

Gene Symbol

TNFSF18

UniProt

Q9UNG2

RefSeq:Q9UNG2