AITRL Antibody

SKU: orb1272727

Description

AITRL Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Indirect:To detect hAITRL by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRLSandwichTo detect hAITRL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human AITRL as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRL. Western Blot:To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAITRL. Human AITRL specific antibody was purified by affinity chromatography employing immobilized hAITRL matrix.
• Target: TNFSF18

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

TL6, AITRL, GITRL, hGITRL, TL6, UNQ149/PRO175, Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand

To detect hAITRL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human AITRL (orb1272726) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRL.

To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained A-431 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

TNFSF18

UniProt

Q9UNG2

RefSeq:Q9UNG2