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Catalog Number | orb345909 |
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Category | Antibodies |
Description | GFP antibody |
Species/Host | Gallus |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IF, IHC, WB |
Reactivity | Other |
Isotype | IgY |
Immunogen | The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
Concentration | 1.17 mg/ml |
Dilution range | ELISA: 1:2,000 - 1:12,000, IHC: 1:200 - 1:1,000, IF: User Optimized, WB: 1:200 - 1:1,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-GFP Antibody IgY purification was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins. |
Conjugation | Unconjugated |
Storage | Store GFP Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | chicken anti-GFP antibody, chicken Anti-Green Fluo Read more... |
Note | For research use only |
Application notes | Polyclonal anti-GFP is designed to detect GFP and its variants. Anti-GFP Antibody has been tested by LEISA, dot blot, and western and is suitable in immunofluorescence. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of KruppleGAL4 driver line in Drosophila eye disc using GFP antibody
Western blot analysis of Lane 1: mouse spleen lysate. Lane 2: mouse spleen lysate spiked with 50 ng of wt GFP. Load: 20 ug per lane using GFP antibody
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