General Vitamin D3 (VD3) ELISA Kit

• Catalog Number: orb1806548
• Category: Assays and Kits
• Description: General Vitamin D3 (VD3) ELISA Kit
• Target: VD3
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin D3(VD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D3(VD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D3(VD3) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 400 ng/mL
• Range: 6.25-400 ng/mL
• Sensitivity: 2.14 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: Cholecalciferol; Toxiferol; Activated 7-Dehydrocho
• Note: For research use only